Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells.
Disruption of this process has been implicated in a variety of diseases such as cancer.
Members of the Bcl-2 family are known to be critical regulators of this process.
These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains.
A related protein termed MAP-1 has recently been identified.
This protein contains a BH3-like domain and induces caspase-dependent apoptosis in mammalian cells when overexpressed.
It forms homodimers and associates with Bcl-2 family members such as Bax, Bcl-2, and Bcl-XL in vitro and in vivo.
It has been suggested that MAP-1 associates with the tumor suppressor RASSF1A following death receptor activation, allowing a conformational change in Bax that leads to cellular apoptosis.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1-4ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:EL4 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human MAP-1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 15aa from near the center of human MAP-1.
