KappaB ras-1 (kB-ras-1) and kB-ras-2 are two small proteins that similar to Ras-like small GTPases that associate with IkappaB (IkB), an inhibitor of the transcription factor NF-kB.
IkB exists in two homologous forms, IkB-a and IkB-B, although IkB-B contains a unique 47aa region within its ankyrin domain.
While inactive IkB-a-NF-kB complexes can shuttle in and out of the nucleus, IkB-B-NF-kB complexes are retained exclusively in the cytoplasm.
It is suggested that kB-ras proteins preferentially bind to the IkB-B form through this unique insert within the ankyrin region, thus modulating the cellular location of IkB-B and regulating the rate of degradation of IkB-k.
This antibody is specific for kB-ras1 and has no cross-reactivity to kB-ras2.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5-2ug/mlImmunocytochemistry: 1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:RAW264.7 Cell Lysate
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human KappaB ras1. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to 15aa from near the C-terminus of human KappaB ras1.
