Mindbomb homolog 1 (MIB1) is an E3 ligase that facilitates the ubiquitination and the subsequent endocytosis of the Notch ligands, Delta and Jagged (1,2).
MIB1 appears to promote the ubiquitination and degradation of death-associated protein kinase (DAPK1) in vitro (3).
Expression of MIB1 is seen in both adult and embryonic murine tissues (4).
Recently, MIB1 was reported to regulate the extrinsic cell death pathway by binding to cellular FLICE-like inhibitory proteins (cFLIP-L and cFLIP-S), which reduces the interaction of caspase-8 with cFLIP and leads to cell death (5).
MIB1 is also involved in T and marginal zone B (MZB) cell development in the lymphopoietic niches (6).
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:100-200Western Blot: 1:100-400Immunocytochemistry: 1:100-500Immunohistochemistry: 1:100-500 (frozen)Immunohistochemistry: 1:50-200 (paraffin sections) Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes rat Mindbomb Homolog 2 (MIB2).
Isotype：IgG
Calc Applications Abbrev：E IC IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant MIB2 (Glu407~Lys663) expressed in E. coli.
