CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes.
CD22 has at least two isoforms (alpha and beta) produced by alternate splicing.
The protein exists predominantly as a monomer of the beta isoform (95kD).
It is also found as a heterodimer of the beta and alpha isoforms.
Applications:Suitable for use in Immunohistochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (formalin fixed, paraffin-embedded): 0.5-1ug/mlWestern Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a powder lyophilized from a solution containing PBS, 5% BSA, 0.05% Thimerosal, and 0.05% sodium azide. Reconstitute in 100ul of sterile dH2O to achieve an antibody concentration of 1mg/ml.
Specificity：Recognizes human CD22.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa 656-670 of human CD22 (UniProt # P20273)
