84-2101-47　UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1) 100ug　145649

特徴

• UTF1 (Undifferentiated embryonic cell Transcription Factor 1) is a 47-49kD member of Leu-zipper family of proteins.
• It is expressed in the early embryonic inner cell mass and primitive ectoderm, and disappears following the formation of the primitive streak.
• In this regard, UTF1 has been shown to be chromatin­associated and to participate in the proper differentiation of embryonic stem cells.
• It also is expressed in basal keratinocytes, and hair follicle matrix cells.
• UTF1 acts as a transcriptional coactivator of ATF2 (Activating Transcription factor 2), a bZIP family protein that interacts with CRE in gene promoters.
• Mouse UTF1 is 399aa in length.
• It contains two conserved domains (aa52-167 and aa269-332), the latter which is key to ATS2 activation.
• There is one alternative start site at Met43 that generates a 39kD isoform.
• Over aa18-423, mouse UTF1 shares 81% and 95% aa sequence identity with human and rat UTF1, respectively.
• Applications:Suitable for use in Flow Cytometry, ELISA, Western Blot, and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Flow Cytometry: 2.5ug/10e6cellsWestern Blot: 0.5ug/ml Immunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.