Human TLR3 is a 116kD type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules.1 TLR3 is found in phagosomes, where the acidic pH enables binding of internalized double-stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells.2-6 Ligand binding by TLR3 induces receptor dimerization, leading to the release of inflammatory cytokines and dendritic cell maturation.3, 4, 6, 8 TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes, and is upregulated by IFN-beta and LPS.4, 8-10 TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection.7 The extracellular domain (ECD) of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for at 12 months at 4°C.
Freezing allophycocyanin conjugates will result in a substantial loss of activity.
Allophycocyaninconjugates are sensitive to light.
仕様
Size：100Tests
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human TLR3.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human Toll-Like Receptor 3, NS0-derived (UniProt # O15455).
