TSLP R, also named Delta and CRLM2 (cytokine receptor-like Module 2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain.1, 2 It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R.3 The TSLP R cDNA encodes a 359 amino acid (aa) residue type I membrane protein with a 24aa residue signal peptide, a 206aa residue extracellular domain that shares 24% sequence identity with the mouse common gamma-chain receptor, a 23aa residue transmembrane domain, and a 106aa residue cytoplasmic domain.
The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with Jaks.
An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported.2 TSLP R expression is ubiquitous in immune and hematopoietic cells, but is upregulated in Th2-skewed cells.
Cells expressing TSLP R alone bind TSLP with low affinity.
Coexpression of TSLP R and IL-7 R alpha is3, 4 required for high-affinity TSLP binding and signal transduction.
Applications:Suitable for use in Flow Cytometry.
Other applications not tested.
Recommended Dilution:Flow Cytometry: 10ul, 1x10e6 cellsOptimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for at 12 months at 4°C.
Freezing allophycocyanin conjugates will result in a substantial loss of activity.
Allophycocyaninconjugates are sensitive to light.
仕様
Size：100Tests
Host：goat
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A or G affinity chromatography.
Form：Supplied as a liquid in PBS, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
Specificity：Recognizes mouse TSLP R.
Isotype：IgG
Calc Applications Abbrev：FC
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa20-233 from mouse TSLP R, NS0-derived.
