Slit2 is a 180210kD secreted member of the SLIT family of proteins.
It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulose lutein cells, and likely keratinocytes.
Slit2 binds to multiple receptors, including ROBO1 thru 4, laminin1, Dan, Gremlin and netrin1.
Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion.
Mature mouse Slit2 is 1496 amino acids (aa) in length (aa261521).
It contains multiple intermingled domains, including nine EGFlike domains, 20 Leurich repeats (LRRs), one laminin Glike and CTCK (Cterminal Cys knotlike) domain, and eight total C plus Nterminal LRRs.
There are two potential isoform splice variants.
One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021.
Slit2 apparently undergoes proteolytic cleavage after Arg1113.
This generates a 140150kD Nterminal protein, and a 5560kD Cterminal fragment.
This processing does not inactivate Slit2.
Rather, it creates molecules with distinct activities.
For example, the Nterminal fragment will bind ROBO1 and repel motor axon migration, while the Cterminal fragment won't bind ROBO1, but will bind glypican1 and promote motor axon migration.
Over aa 26900, mouse SLIT2 shares 99% and 97%aa sequence identity with rat and human Slit2, respectively.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 515ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes mouse Slit2 in direct ELISAs. Species Crossreactivity: <5% crossreactivity with recombinant mouse (rm) Slit1 and rmSlit3.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to aa26-900 from mouse Slit2, CHO-derived (UniProt # Q9R1B9).
