84-2101-37　SEMA4A (Semaphorin 4A, CORD10, SEMAB, SEMB) 100ug　145602

特徴

• Sema4A (Semaphorin 4A; also Sema B) is a class IV member of the semaphorin family of proteins.
• It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells.
• It reportedly binds to Tim­2 and Plexin D1 and B1.
• In the immune system, it serves to activate naïve T cells and induce a Th1 phenotype.
• In the nervous system, it induces growth cone collapse in neurons.
• Mature human Sema4A is an 80kD (predicted) type I transmembrane glycoprotein that is 729 amino acids (aa) in length (aa33­761).
• It contains a 651aa extracellular region (aa33­683) that is characterized by the presence of one Sema domain (aa 36­494), a PSI region (aa496­528), and an Ig­like C2­type domain (aa573­631).
• The 57 aa cytoplasmic region contains two potential phosphorylation sites at Thr744 and Ser747.
• There are two potential splice variants.
• One contains a 37aa substitution for aa293­328, while a second contains an alternative start site at Met100 coupled to a deletion of aa122­154.
• Sema4A is reportedly cleaved to generate both a monomeric and a dimeric soluble isoforms.
• Over aa 32­683, human Sema4A shares 86% aa identity with mouse Sema4A.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.