84-2101-36　RPS6 (Ribosomal Protein 56, Phosphoprotein NP33) 100ug　145598

特徴

• (40S) Ribosomal protein S6 (rpS6; also Phosphoprotein NP33) is a 32­34kD member of the ribsosmal protein S6e family of molecules.
• It is ubiquitously expressed and involved in the regulation of cell size.
• Higher eukaryotic ribosomes contain a small (or 40S) and large (or 60S) subunit.
• The small subunits are composed of one RNA molecule plus more than 30 individual proteins, one of which is rp6S.
• It is a substrate of both PASKIN and S6 kinase, and when phosphorylated C­terminally, appears to slow the rate of protein synthesis.
• Notably, the activities of rpS6 are complex, as phosphorylation seems to decrease cell proliferation and glucose utilization on one hand, while increasing cell size on the other.
• Human ribosomal protein S6 is 249 amino acids (aa) in length.
• It possesses two acetylated lysines plus six phosphorylated serines.
• There are three potential splice forms.
• One contains a four aa substitution for aa1­46, a second utilizes an alternative start site at Met32, and a third utilizes the same Met32 start site coupled to a substitution of a Gln residue for aa234­243.
• Full­length human, mouse and rat rpS6 are identical in their amino acid sequences.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.