84-2101-33　PTGIS (Prostaglandin I2 Synthase, CYP8, CYP8A1, PGIS, Prostacyclin Synthase, PTGI) 100ug　145589

特徴

• PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50­52kD (57kD predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules.
• It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium.
• Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2).
• Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS.
• Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation.
• It apparently does so by binding to either the IP receptor or PPARγ.
• Human PTGIS is 500 amino acids (aa) in length.
• It is a single span transmembrane protein that contains an N­terminal transmembrane segment (aa1­20) coupled to a lengthy 480aa cytoplasmic region.
• Over aa21­500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.