84-2101-30　PRCP (Angiotensinase C, HUMPCP, Prolylcarboxypeptidase, PCP, Lysosomal Pro-X Carboxypeptidase, Lysosomal Carboxypeptidase C) 100ug　145578

特徴

• PRCP (Prolylcarboxypeptidase; also PCP, lysosomal Pro­X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57­62kD glycoprotein member of the S28 family of proteases.
• It is expressed by a number of cells, including fibroblasts, macrophages, and endothelial cells.
• PRCP cleaves a variety of single C­terminal amino acids (aa) adjacent to a Pro residue, and is known to act on, and inactivate, peptides such as prekallikrein, alpha­MSH and angiotensin II plus III.
• Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa1­21), a short prosequence (aa22­45) and a 451aa mature region (aa46­496).
• The mature region demonstrates a Ser­carboxypeptidase domain (aa55­477) that, in itself, possesses an SKS domain that caps the enzyme's catalytic site.
• The molecule is known to be mannosylated, and to form noncovalent homodimers.
• There is one splice variant that contains a 21 aa insertion after Lys56.
• Over aa1­496, human and mouse PRCP share 77% aa sequence identity.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.