PIKfyve (PhosphoInositide Kinase for five position containing a fyve finger (FAB1/YOTB/Vac1/EEA1) domain; also phosphatidylinositol 3phosphate 5kinase type III and Type III PIP kinase) is a 230260kD cytoplasmic and endocytic member of an ancient family of phosphatidylinositol3,5 biphosphate (PI[3,5]P2 synthesizing enzymes.
It is associated with endosomes, particularly the microdomains of early endosomes that are EEA1deficient.
PIKfyve binds to membraneembedded phosphatidylinositol and initially converts PI3P into PI(3,5)P2, which is then converted into PI5P.
In conjunction with ArPIKfyve and Sac3, its actions are involved in the normal transport, fusion, and membrane export of endosomes.
Notably, PIKfyve also appears to act as a protein kinase, apparently controlling its own activity through autophosphorylation.
Human PIKfyve is 2098 amino acids (aa) in length.
It contains one FYVE PI3Pbinding zincfinger domain (aa154219), a DEP domain (aa365440) and a Cterminal PIP kinase rehion (aa11722085).
There are no less than 29 utilized Ser/Thr phosphorylation sites, plus two utilized Tyr phosphorylation sites.
Three potential isoform variants are reported, all which possess a three aa substitution for aa5462098.
Two of these three also demonstrate a deletion of aa108204, and a nine aa substitution for aa109203, respectively.
Over aa215361, human and mouse share 95% aa sequence identity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human PIP5K3 in direct ELISAs and Western blots.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa215-361 from human PIP5K3, expressed in E. coli (UniProt # Q9Y2I7).
