84-2101-28　PHD2 (Prolyl Hydroxylase Domain-containing Protein 2, C1orf12, HIFPH2, HPH2, SM20, ZMYND6, EGLN1) 100ug　145568

特徴

• PHD2 (Prolyl Hydroxylase Domain-containing protein 2; also HPH2, EGLN1 and HIF-PH2) is a 45-47kD dioxygenase member of the PH family of enzymes.
• It is ubiquitously expressed, and serves to regulate the availability of the oxygen-sensitive HIF transcription factor.
• Active HIF1alpha is a heterodimer of alpha- and beta-subunits and when intact, promotes VEGF and EPO production.
• The beta-subunit is constitutively expressed, while alpha-subunit levels are regulated by intracellular oxygen concentration.
• At normoxic levels, the alpha-subunit is hydroxylated on Pro by one of three PHDs, inducing its ubiquitination/degradation.
• The hydroxylation event requires oxygen, and thus PH activity (particularly PHD2) is a measure of a cell's oxygen concentration.
• Human PHD2 is 426 amino acids (aa) in length.
• It contains an NES (aa6-20), a Zn-finger region (aa21-58), and a catalytic domain (aa291-392).
• There are five nitrosylated cysteines plus one acetylated alanine.
• Two isoform variants are known, one that shows a deletion of aa338-359, and another that contains a 17aa substitution for aa58-175.
• Over aa157-426, human PHD2 shares 93% aa sequence identity with mouse PHD2.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.