NQO2 (NADPH Quinone acceptor Oxidoreductase 2; also QR2, melatonin receptor 3/MT3 and NRH dehydrogenase) is a 2526kD cytoplasmic flavoprotein member of the NAD(P)H dehydrogenase family of enzymes.
It shows restricted expression, being found in retinal pigment epithelium, prostatic fibroblasts, select CNS neurons and RBCs.
Like NQO1, NQO2 would appear to reduce catechol quinones, but without the use of NADH.
In contrast to NQO1, NQO2 would appear to activate some quinones, generating cytotoxic products.
NQO2 also reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosomemediated protein turnover.
Finally, NQO2 reportedly binds to melatonin, an act that may promote the subsequent binding of the androgen receptor and an inhibition of cell proliferation.
Human NQO2 is 231 amino acids (aa) in length.
It contains a flavodoxinlike domain (aa4212) and three utilized Ser phosphorylation sites.
NQO2 functions as a noncovalent homodimer.
There are two potential isoform variants.
One shows an alternative start site at Met117, while another shows a deletion of aa102139.
Full length human NQO2 shares 81% aa sequence identity with mouse NQO2, and less than 50% aa sequence identity with human NQO1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human NQO2 in direct ELISAs and Western blots. Species Crossreactivity: <3% with recombinant human NQO1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa2-231 from human NQO-2 expressed in E. coli (UniProt # P16083).
