NQO-1 (NADPH Quinone acceptor Oxidoreductase 1; also DTD and Menadione reductase) is a 31-33kD cytoplasmic member of the NAD(P)H dehydrogenase family of enzymes.
It is widely expressed, being found in adipocytes, endothelial cells and hepatocytes.
NQO-1 serves as a cytoprotective molecule, reducing quinones (atmospheric benzene and esrtrogen compounds) and nitroaromatics without consuming sulfhydryl compounds.
It also acts as a backup superoxide scavenger to SOD.
NQO-1 reportedly binds and stabilizes key cell protection molecules such as p53, thus acting as a gatekeeper for proteosome-mediated protein turnover.
Human NQO-1 is 274 amino acids (aa) in length.
It contains an FMN reductase domain (aa5-212) and two utilized Lys acetylation sites.
NQO-1 functions as a noncovalent homodimer.
There are three potential at Met22, while two others show a deletion of aa141-174 and 102-139, respectively.
Full length human NQO-1 shares 86% aa sequence identity with mouse NQO-1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human NQO-1. Species Crossreactivity: In direct ELISAs, <1% with recombinant human NQO2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa1-274 from human NQO-1, expressed in E. coli (UniProt # P15559).
