Neuroplastin (NPTN or neural plasticity-dependent synaptic rearrangement protein; also Np65 and SDR-1) is a 63-67 kDa member of the CD147 family, Ig‑superfamily of molecules.
Its expression is primarily restricted to the PSD of select neurons, and it likely serves as a cell adhesion molecule between cells.
NPTN is known to bind homotypically in trans, and this complex appears to interact with FGFR1 in cis.
Engagement of the FGFR induces neurite outgrowth.
Notably, rodent and human Np65 show distinctly different staining patterns in the hippocampus and cerebellum of the brain.
Mature mouse NPTN is a 369 amino acid (aa) type I transmembrane glycoprotein.
It possesses a 310 aa extracellular region (aa 29-338) that contains three Ig-like domains (aa 29-134; 148-234; 237-327), and a 38 aa cytoplasmic tail.
There are three splice forms that have been described.
One is a widely-expressed 53-57 kDa isoform (Np55) that contains a Glu substitution for aa 31‑147, a second is a splice form that shows the above substitution coupled to a deletion of aa 371-374, and a third is an isoform that contains a nine aa substitution for aa 1-235.
Over aa 1-338, mouse NPTN shares 95% and 98% aa sequence identity with human and rat NPTN/Np65, respectively.
Applications:Suitable for use in ELISA, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.05ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Neuroplastin in direct ELISAs and Western blots. Species Crossreactivity: ~100% recombinant human Neuroplastin65.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa29-338 from mouse Neuroplastin, NS0-derived (UniProt # P97300).
