84-2101-23　NOXA2 (NCF2, p67phox, 67kD Phagocyte Oxidase, NADPH Oxidase Activator 2) 100ug　145552

特徴

• P67phox (67kD phagocyte oxidase; also NCF2 and NADPH oxidase activator 2) is a 67­70kD cytosolic member of the NCF2/NOXA1 family of proteins.
• It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts.
• Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes.
• This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane­associated cytochrome b558, plus Rac GTPase and three SH3 domain­containing components, p47phox and a p67phox:p40­phox dimer.
• Human p67phox is 526 amino acids (aa) in length.
• It contains two TPR repeats that participate in scaffold formation (aa11­98), an SH3 domain (aa240­299), an OPR motif that regulates PB1 interactions (aa51­429), and a C­terminal SH3 domain (aa457­516).
• There is one splice variant that shows a deletion of aa231­293, and multiple point mutations along the length of the molecule.
• Over aa355­526, human p67phox shares 76% aa identity with mouse p67phox.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.