Nicalin (NICAstrin-Like proteIN) is a 60-62kD member of the nicastrin family of proteins.
It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons.
Nicalin interacts with 22kD TMEM147 and 130kD NOMO1 to form a 200-220kD complex that antagonizes Nodal signaling.
Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein.
It contains a 480aa luminal/extracellular region (aa 43-522) plus a 20aa cytoplasmic domain (aa544-563).
The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa130-429).
Four potential isoform variants are reported.
One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102aa substitution for aa162-563, and a fourth shows a deletion of Gln446.
Over aa1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A or G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human Nicalin. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa1-522 from human Nicalin, S. frugiperda insect ovarian cell line Sf 21-derived UniProt # Q969V3).
