84-2101-21　Netrin-1 (NTN1) 100ug　145547

特徴

• Netrin­1 (from Sanskrit netr meaning "to guide"; also known as Epididymis tissue protein Li 131P) is a 66­76kD secreted member of the laminin superfamily of molecules.
• It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia.
• Netrin­1 has multiple receptors, including beta 4­integrins, DCC, DSCAM and UNC5A­thru­D.
• Its effects are context specific.
• For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion.
• In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis.
• Mature human netrin­1 is 580 amino acids (aa) in length (aa25­604).
• It contains an N­terminal laminin domain (aa47­284), three EGF­like domains (aa285­453) and a C­terminal cell­interaction netrin­like domain (aa472­601).
• Over aa22­604, human netrin­1 shares 99% aa sequence identity with mouse netrin­1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.