84-2101-20　Mx1 (Interferon-regulated Resistance GTP-binding Protein MxA, MxA, IFI78, IFI-78K, OrthoMyxovirus-resistant Protein A, IFN-induced Protein p78/IFI-78K, Mx1) 100ug　145535

特徴

• MxA (orthoMyxovirus­resistant protein A; also IFN­induced protein p78/IFI­78K and Mx1) is a 75­78kD member of the Dynamin family, GTPase­binding superfamily of proteins.
• It is induced by type I and III interferons, and is known to reside in the cytoplasm and associate with the membranes of the ER.
• Typically, IFN­alpha is produced when influenza virus RNA (plus other viral dsDNA and RNA) is detected by internal Pattern recognition receptors (PRRs).
• IFN­alpha induces the synthesis of 300+ genes, one of which is MxA (Mx1 in mouse).
• In primates, MxA is cytoplasmic, and interacts with the influenza virus replication cycle at a point proximal to the transcription of genomic RNA into mRNA.
• By contrast, Mx1 in mouse acts in the nucleus to block primary viral gene transcription.
• Functionally, MxA is a GTPase, but it is only the binding of GTP to MxA that is associated with antiviral activity.
• Nucleotide binding to MxA is also associated with nonviral activity, being associated with the potentiation of TRPC Ca2+ channel activity.
• Human MxA is 662 amino acids (aa) in length.
• It contains one GTPase domain (aa69­340) and a GED (GTPase Effector Domain) over aa574­662.
• There are two ultilized phosphorylation sites at Tyr129 and Tyr451.
• MxA forms homo­dimers, ­tetramers and ­oligomers, with multimerization suggested to be important for activity.
• Although IFNs are typically considered to induce Mx gene expression, HSV­1 itself also activates gene transcription.
• In this case, however, a truncated 54­57kD transcript is generated that contains an 84 aa substitution for aa425­662.
• Over aa412­630, human MxA shares 49% aa sequence identity with mouse Mx1.
• Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1ug/ml Human peripheral blood mononuclear cells (PBMC) treated with Recombinant Human IFN‑αUniversal Type I and LPSImmunohistochemistry: 5­-15ug/ml Immersion fixed paraffin-embedded sections of human thymusImmunocytochemsitry: 1-15ug/ml Immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with IFN-alphaOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.