84-2101-18　MKI67 (Ki-67, KIA, TSG126) 100ug　145526

特徴

• MKI67 (also Ki-67) is a 350-400kD nuclear protein that belongs to a molecular group comprised of mitotic chromosome-associated proteins.
• Ki-67 was originally recognized as an antigen associated with the monoclonal Ki-67 antibody raised against Hodgkin's lymphoma nuclear material.
• Ki-67 is contextually expressed, being potentially found in all cells that are not in the Go phase of the cell cycle.
• Thus, MKI67 qualifies as a cell proliferation marker.
• Functionally, Ki-67 is known to interact with 160kD Hklp2, a protein that promotes centrosome separation and spindle bipolarity.
• It also directly interacts with NIFK, and apparently binds to UBF, thus playing a role in rRNA synthesis.
• Human MKI67 is 3256 amino acids (aa) in length.
• It contains one FHA domain (aa 8-98), followed by at least 24 utilized Ser/Thr phosphorylation sites and sixteen 120aa repeats (aa 1000-2928) that are interspersed with at least 90 additional utilized phosphorylation sites.
• There are two potential isoform variants.
• One isoform is 315-345kD in size and shows a deletion of aa136-495, while a second isoform contains a 58 aa substitution for aa1-513.
• Over aa3120-3256, human Ki-67 shares 46% aa sequence identity with the mouse ortholog to Ki-67.
• Applications:Suitable for use in ELISA and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.