MFN1 (Mitofusin 1; also Fzo/"fuzzy onions" homolog and Transmembrane GTPase MFN1) is an 8090kD mitochondrial member of the dynamin family of molecules.
It is ubiquitously expressed, and found in the outer mitochondrial membrane.
MFN1 apparently mediates the fusion of individual mitochondria with each other through transinteractions with adjacent mitochondrial MFN1 and/or MFN2.
Pertubations with this process result in both a general overall fragmentation of mitochondria, and in cellspecific effects such as a reduction in oxidative phosphorylation or an imbalance in the generation of ROS.
MFN1 has two key domains.
One is a coiledcoil region that mediates MFN1:MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion.
Human MFN1 is a 741 amino acid (aa) two transmembrane protein that contains a cytoplasmic N and Cterminus.
The cytoplasmic Nterminus (aa1584) contains the GTPase domain (aa82286) while the cytoplasmic Cterminus possesses a critical coiledcoil motif (aa679734).
MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5.
Multiple splice variants are reported to occur.
One is cytosolic and contains a four aa substitution for aa367741.
Three others show a deletion of aa444554, aa326408 and an alternative start site at Met387, respectively.
Over aa177, human and mouse MFN1 share 88% aa sequence identity.
Fulllength human MFN1 and MFN2 share 61% aa sequence identity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human Mitofusin 1 in direct ELISAs and Western blots. Species Crossreactivity: <3% with recombinant human Mitofusin 2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to aa2-77 from human Mitofusin 1, expressed in E. coli (UniProt # Q8IWA4).
