84-2101-16　MIF (Macrophage Inhibitory Factor, GIF) 100ug　145523

特徴

• MIF (Macrophage Inhibitory Factor; also GIF) is a 12-13kD member of the MIF family of proteins.
• Although secreted, it possesses no definitive signal sequence.
• MIF is widely expressed, and serves to regulate immune reactions.
• On macrophages and B cells, MIF binds to a CD74:CD44 complex, initiating downstream signaling.
• On monocytes, this promotes TNFxalpha production plus IFN-gamma-stimulated NO production.
• On B cells, this promotes B cell survival.
• MIF also binds to leukocyte CD74:CXCR2 and CD74:CXCR4 heterodimers, initiating T cell and monocyte migration.
• A tautomerase activity has also benn shown for MIF, but the significance of this is unclear.
• Mouse MIF is 114 amino acids (aa) in length.
• Its tautomerase activity is suggested to involve the entire length of the molecule.
• MIF is phosphorylated at Ser91, and an additional, singular Cys residue is covalently linked to MIF at Cys60.
• MIF is considered to act as a homotrimer.
• Full-length mouse MIF shows 90% and 99% aa sequence identity with human and rat MIF, respectively.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.