MFN-2 (Mitofusin 2; also Hypertension-related protein 1, Mitochondrial assembly regulatory factor, HSG protein, and Transmembrane GTPase MFN2) is an 85-100kD mitochondrial member of the dynamin family of molecules.
It is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane.
Through transinteractions with MFN-1 and/or MFN-2, MFN-2 apparently mediates the fusion of individual mitochondria with either the ER or with adjacent mitochondria.
Pertubations with this process result in both a general failure of mitochondria to fuse, and in cell-specific effects such as a reduction in oxidative phosphorylation, a decrease in the axonal transport of mitochondria, and a deficit in the expression of respiratory chain components.
MFN-2 has two key domains.
One is a coiled-coil region that mediates MFN-2:MFN-1/2 binding, and a second is a GTPase domain that likely plays a role in fusion.
While active, the MFN-2 GTPase domain is 8fold lower in activity than that for MFN-1.
Human MFN-2 is a 757 amino acid (aa) two transmembrane protein that contains a cytoplasmic N and C-terminus.
The cytoplasmic N-terminus (aa1-604) contains the GTPase domain (aa99-258) while the cytoplasmic C-terminus possesses a critical coiled-coil motif (aa696-738).
MFN2 is known to form oligomers, either with itself or MFN1.
Two potential splice variants are reported, one that shows a deletion of aa245-273, and a second that contains a 33aa substitution for aa573-757, suggesting this might be a soluble for of MFN-2.
Over aa364-599, human and mouse MFN-2 share 93% aa sequence identity.
Full-length human MFN-2 and MFN-1 share 61% aa sequence identity.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse skeletal muscleOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human Mitofusin 2. Species Crossreactivity: mouse and rat Mitofusin 2, <1% recombinant human Mitofusin 1.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Arg364-Phe599 from human Mitofusin 2, expressed in E. coli
