MARCO (Macrophage receptor with collagenous structure; also scavenger receptor class A member 2/SR-AII) is a 65-70kD pattern recognition receptor/PRR that belongs to class A of the SR family, SRCR superfamily of molecules.
It is expressed by macrophages, monocytes and Kupffer cells, and apparently binds to a number of microbes, including Gram+ bacteria (Staph and Strep) and Gram- bacteria (Neisseria and coliforms), plus crystalline silica.
Human MARCO is a 520 amino acid (aa) type II transmembrane glycoprotein.
It contains a 43 aa cytoplasmic region and a 456 aa extracellular domain/ECD (aa 65-520).
The ECD possesses one collagen-like region (aa 147-419) and an SRCR domain (aa 424-519).
Human MARCO is believed to form trimers and/or multimers, and these are likely noncovalent associations.
Over aa 79-520, human and mouse MARCO share 70% aa sequence identity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25ug/ml Lysates of THP1 human acute monocytic leukemia cell line, human lung tissue and human liver tissue Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes human MARCO in direct ELISAs and Western blots. Species Crossreactivity: ~10% recombinant mouse MARCO.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Met79-Val520 from human MARCO, expressed in CHO cells.
