LCN-13 (Lipocalin 13; also Odorant-binding protein 2a) is a secreted, 26-28 kDa member of the LCN family, calycin superfamily of molecules.
Its name is derived from Greek, and denotes the fact that lipocalins are known to be "fat-wrapping", or fat binding proteins that interact with small lipophilic molecules within a central cavity.
LCN-13 is expressed by hepatocytes and smooth muscle cells.
It is known to have antidiabetogenic effects, including the suppression of hepatic glucose production, the uptake of circulating glucose by adipocytes, and the induction of fatty acid oxidation by liver, possibly by stimulating CPT1 alpha production.
Mouse LCN‑13 is synthesized as a 176 amino acid (aa) precursor that contains a 19 aa signal sequence and a 157 aa mature region.
Although no splice forms have been reported for LCN-13 to date, a smaller, presumably proteolytically processed 23-26kD isoform has been noted on SDS-PAGE.
Mature mouse LCN-13 shares 69% and 39% aa sequence identity with rat LCN-13 and human odorant-binding protein 2b, respectively.
Applications:Suitable for use in Direct ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity：Recognizes mouse Lipocalin13.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to Ala19-Asp176 from mouse Lipocalin-13, expressed in E. coli.
