Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins.
It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm.
In the adult, Lefty-2 appears in oviduct epithelium.
Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity.
Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan.
Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor.
It contains a 21aa signal sequence, plus a 347aa, 41-42kD bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites.
If cleavage occurs after Arg77, the resulting 33-34kD mature form (aa78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28kD mature form (aa136-368) is biologically active.
Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer.
Over aa78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlImmunocytochemistry: 5-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes mouse Lefty-2 in direct ELISAs and Western blots. Species Crossreactivity: In direct ELISAs, approximately 65% with recombinant mouse Lefty-1, and approximately 20% with recombinant human Lefty-2.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa78-368 from Lefty-2, CHO-derived (UniProt # P57785).
