HDAC2 (Histone deacetylase 2; also HD2) is a 55-60kD nuclear member of the class I subfamily, histone deacetylase family of transcriptional regulators.
It is found in a variety of cell types including monocytes, cardiac muscle cells and neurons.
HDAC2 is also crucial for embryonic development.
HDAC2 catalyzes the hydrolytic release of acetyl groups from acetylated histone proteins.
Within the context of a large complex, this has the effect of repressing gene activity.
Human HDAC2 is 488 amino acids (aa) in length.
It contains one histone deacetylation domain (aa29-388), plus sites for phosphorylation, nitrosylation, ubiquitination, and nitration.
There is one alternative start site at Met31.
Over aa386-488, human HDAC2 shares 98% and 53% aa sequence identity with mouse HDAC2 and human HDAC1, respectively.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A or G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human, mouse, and rat HDAC2 in Western blots. Species Crossreactivity: In direct ELISAs, <2% with recombinant human HDAC1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa386-488 from human Histone Deacetylase 2/HDAC2, expressed in E. coli.
