Glis1 (GLI/gliomaSimilar 1; also GliH1) is a 66kD (predicted) member of the Kruppellike zinc finger family of proteins.
In the adult, it is principally expressed in placenta and kidney, while in the embryo, it has widespread expression in mesodermal structures.
Glis1 can be induced in suprabasal keratinocytes by activating cytokines, and is believed to promote a unique differentiation program.
Glis1 is also effective in generating iPSCs from somatic cells (fibroblasts) when concurrently expressed with Oct3, Sox2 and Klf4.
Multiple reprogramming pathways are involved, and include Myc, Wnt and Nanog molecules.
Functionally, Glis1 is believed to act as a transcriptional repressor.
Although it has been described as possessing an activation domain, it would appear that gene promotion is a consequence of the repression of a "repressor".
The consequence sequence ACCACCCA corresponds to a GLIS binding motif.
Human Glis1 is 620 amino acids (aa) in length.
Based on mouse, it contains a zincfinger repressor region (aa197346) plus a putative transactivation domain (aa 448620).
And again based on mouse, there might be an ~796aa splice form that shows an alternative start site 171aa upstream of the 620aa isoform.
The existence of such an isoform would generate an ~95100kD isoform in SDSPAGE.
Over aa446620, human Glis1 shares 80% aa sequence identity with mouse Glis1.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human Glis1 in direct ELISAs and Western blots. Species Crossreactivity: ~60% with recombinant mouse Glis1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa446-620 from human Glis1, expressed in E. coli (UniProt # Q5VTL4).
