GCN2 (General Control Non-derepressible 2-like protein; also eIF2a kinase 4) is a 190-200kD member of the GCN2 subfamily, Ser/Thr kinase family, protein kinase superfamily of proteins It is widely expressed, being found in skeletal muscle cells, fibroblasts, hepatocytes and neurons.
GCN2 is a key regulator of metabolism under nutrient deprivation.
In particular, when essential amino acids (aa) are limiting, tRNAs exist that are unbound to amino acids.
These “uncharged” tRNAs will bind to GCN2, activating the molecule.
Through GCN2-mediated phosphorylation of eIF2a, promotes GCN4 synthesis, with subsequent activation of amino acid synthesizing enzymes, and a downregulation of lipid storage.
Human GCN2 is 1649aa in length.
It possesses one RWD domain (aa25-137), two protein kinase domains (aa296-539 and 590-1001) and an extended His-RS-like region (aa1021-1492).
There are at least three utilized Ser/Thr phosphorylation sites.
Two isoform variants exist.
One shows a deletion of aa774-801, while another contains a nine aa substitution for aa608-1649.
Over aa22-139, human GCN2 shares 91% aa sequence identity with mouse GCN2.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A or G affinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human GCN2 when phosphorylated at T899 in Western blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Phosphopeptide containing human GCN2 T899 site.
