GALNT2 (NAcetylGalactoseaminyl Transferase 2; also UDPAcetylgalactosaminyltransferase 2 and ppGalNAcT2) is a 6874kD member of the GalNAC transferase subfamily, glycosyltransferase 2 family of enzymes.
It is widely expressed, being found on basal keratinocytes, hepatocytes, B cells, renal tubular epithelium, and virtually all cell lines examined to date.
GALNT2 is found in the Golgi apparatus, and catalyzes the transfer of UDPGalNAC onto either a Ser or Thr residue on a previously glycosylated peptide/polypeptide backbone.
The generation of Olinked carbohydrates is believed to play a role in cytokine proteolytic processing, as the presence of Olinked sugar adjacent to a PC processing site is known to inhibit proteolysis and molecule inactivation.
Human GALNT2 is a 571 amino acid (aa) type II transmembrane protein.
It contains a six aa Nterminal cytoplasmic region and a 547aa extracellular domain (aa25571).
The ECD possesses two key parts, a catalytic region with two catalytic subdomains (aa135240 and 300362), and a ricintype lectin domain that binds carbohydrates (aa456566).
The latter domain is suggested to facilitate GALNT2 action by imparting specificity and stability to the overall enzyme activity.
A 52kD soluble form of GALNT2 has been reported that begins at Lys52.
There are two potential splice form variants.
Both contain a four aa substitution for aa142, and one contains an additional four aa substitution for aa543571.
Over aa52571, human GALNT2 shares 97% aa sequence identity with mouse GALNT2.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human Polypeptide GalNac Transferase 2/GALNT2 in direct ELISAs and Western blots. Species Crossreactivity: <1% recombinant human (rh) GALNT1 and rhGALNTL1.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human GALNT2, NS0-derived (UniProt # Q10471).
