FCRLA (Fc ReceptorLike A; also known as FcRX, FcRL1 and Fc receptor Homolog Expressed in B cells/FREB) is a 4044kD intracellular member of the Fc gamma RI class, FcR family, Immunoglobulin Superfamily of molecules.
Initially, it is associated with germinal center B cells that are undergoing somatic hypermutation and classswitch recombination.
Now, it is believed that all B cells (but not CD38+ plasma cells) are also FCRLA+.
FCRLA is suggested to act as an ER chaperone during antibody maturation, and is known to bind to IgM, IgA, and IgG prior to their secretion.
Human FCRLA is synthesized as a 359 amino acid (aa) precursor that contains a 27aa signal sequence plus a mature region that contains two C2type Iglike domains (aa70159 and 170257), and a Cterminal polyProline region (aa269315).
Although there is no traditional ER retention signal, a viable substitute is assumed to exist in the Nterminus of the mature molecule.
FCLRA is believed to exist naturally as a monomer; however, disulfidelinkage can occur during experimental manipulation.
At least eight potential isoform variants have been reported.
It is unclear if, or how frequently they are expressed.
One utilizes an alternative start site 17aa upstream of the standard site, a second contains a six aa insertion after Ala28, and a third possessess an four aa substitution for aa29166.
Five show single block deletions; in random order, they involve aa78166, 27166, 78261, 21261 and 16726.
Over aa270359, human FCLRA shares 55% aa sequence identity with mouse FCRLA.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute in sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human FCRLA in direct ELISAs and Western blots. Species Crossreactivity: ~510% with recombinant mouse FCRLA.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to aa270-359 from human FCRLA, expressed in E. coli (UniProt # Q7L513).
