COTL1 (Coactosin-like Protein) is both a cytoplasmic and plasmaappearing 15-16kD member of the coactosin subfamily, ADF/Actin Depolymerizing Factor family of actin-binding proteins.
It is widely expressed, and found in cell types such as neutrophils, and tissues such as placenta, lung and kidney.
Functionally, COTL1 interacts noncovalently with both F-actin and 5lipoxygenase/5LO.
These interactions appear to be mutually exclusive.
A COTL1:Factin interaction leads to actin binding without actin polymerization, while a 5LO:COTL1 interaction has two potential outcomes; first, 5LO sequesters COTL1, leading to a failure of actin binding, and second, COTL1 can serve as a scaffold for 5LO activity, facilitating the production of either 5HPETE or LTA4.
Human COTL1 is 142 amino acids (aa) in length.
It is principally composed of one ADF-H domain (aa2-130) that possesses a utilized phosphorylation site at Ser115, and two acetylation sites at Lys102 and Lys126.
COTL1 may form noncovalent homodimers and oligomers, but not when complexed to Factin.
There is one potential isoform variant that shows a 106aa substitution for aa1-53.
Full-length human and mouse COTL1 share 95% aa sequence identity.
Applications:Suitable for use in Direct ELISA, Western Blot, and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1ug/ml using JAR human choriocarcinoma cell line, NCL-H345 human small cell lung carcinoma cell line, human placenta tissue and rat placenta tissueImmunocytochemistry: 5-15ug/ml using immersion fixed NCI-H128 human small cell lung carcinoma cell lineOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in 500ul sterile PBS.
Specificity：Recognizes human COTL1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to Ala2-Glu142 from human COTL1, expressed in E. coli.
