84-2100-92　CCBL1 (Glutamine Transaminase K, KAT1, KATI, KAT-I, Kyneurenine Aminotransferase I, Cysteine Conjugate beta­Lyase 1, GTK) 100ug　145407

特徴

• CCBL1 (Cysteine Conjugate beta­Lyase 1; also GTK and KAT­I) is a 90­120kD homodimeric member of the Class I Pyridoxyl­phosphate­dependent aminotransferase family of enzymes.
• It is widely expressed, being found in fibroblasts, renal epithelium, hepatocytes, neurons and astrocytes.
• CCBL1 has multiple activities, including converting prodrugs into active formulations, generating kynurenic acid from kynurenine, thereby antagonizing NMDA receptors (but not likely presynaptic alpha 7 AChRs), and blocking the release of proinflammatory cytokines by microglia via GPCR­35 antagonism.
• Human CCBL1 is 422 amino acids (aa) in length, and contains one aspartate aminotransferase­like region (aa31­417).
• There are three potential splice variants, one that shows a deletion of aa68­117, a second that shows a deletion of aa251­422, and a third that contains a 21aa substitution for aa230­250.
• While the 422aa isoform is considered cytosolic, there is the potential for a 34aa N­terminal extension that would act as a mitochondrial targeting sequence.
• Such a sequence is reported for rat and nonhuman primate.
• Over aa182­422, human CCBL1 shares 82% aa sequence identity with mouse CCBL1.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months at -20°C.
• Reconstitute with sterile ddH2O or PBS.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.