LMWPTP (Low Molecular Weight Protein Tyrosine Phosphatase; also Red cell acid phosphatase 1/ACP1, HCPTP, and HAAP/Adipocyte acid phosphatase) is a 1718kD member of the LMWPTP family, PTP superfamily of enzymes.
It is widely expressed, being found in cells diverse as B and T cells, endothelial cells and vascular smooth muscle cells.
LMWPTP dephosphorylates multiple substrates, often in a celldependent manner.
Among the substrates for LMWPTP include PDGR beta, EphA2 and betacatenin.
PMWPTP is found intracellularly, generally associated with two cytoplasmic pools, one in the cytosol and the other accompaning the cytoskeleton.
Human LMWPTP is 158 amino acids (aa) in length.
The catalytic region spans aa9154, and there are two acetylation sites at Ala2 and Lys156.
Phosphorylation on Tyr132 and Tyr133 occurs on cytoskeletonassociated LMWPTP, and has the effect of increasing its phosphatase activity.
There are three alternative splice variants reported for LMWPTP.
One shows a 34aa substitution for aa4174, a second shows a deletion of aa4174, while a third contains a 35aa substitution for aa78158.
The last two isoforms may function a negative regulators of LMWPTP activity.
Fulllength LMWPTP shares 86% aa sequence identity with mouse LMWPTP.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O or PBS.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute in sterile PBS.
Specificity：Recognizes human, mouse, and rat LMWPTP/ACP1 in Western blots.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to aa2-158 from human LMW‑PTP/ACP1, expressed in E. coli (UniProt # P24666).
