In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton.
By linkage studies in a 3-generation family, Mulligan et al.
(1992) mapped the VCL gene to chromosome 10q22.1-q23, distal to D10S22.Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane.
Multiple alternatively-spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Human vinculin protein exhibits a greater-than-95% sequence identity to the chicken vinculin protein.Vinculin is a 117-kDa cytoskeletal protein with 1066 amino acids.
The protein contains an acidic N-terminal domain and a basic C-terminal domain separated by a proline-rich middle segment.
Vinculin consists of a globular head domain that contains binding sites for talin and alpha-actinin as well as a tyrosine phosphorylation site, while the tail region contains binding sites for F-actin, paxillin, and lipids.
UniProt Number:P18206
Gene ID:VCL
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes mouse Vinculin/VCL. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human VCL, identical to the related rat and mouse sequences.
