TOLLIP (TOLL-Interacting Protein), is an inhibitory adaptor protein that in humans is encoded by the TOLLIP gene.
Lo et al.
(2009) stated that the TOLLIP gene maps to chromosome 11.
The mouse gene maps to chromosome 7.
By Western blot analysis of embryonic kidney cells, Burns et al.
(2000) confirmed the binding of TOLLIP to IL1RAP, to a complex of IL1RAP-IL1R1, and to IL18R.
Burns et al.
(2000) proposed that IL1B stimulation induces aggregation of IL1Rs, recruitment of MYD88 followed by TOLLIP-IRAK complexes, and the phosphorylation of IRAK by MYD88.
This leads to the dissociation of TOLLIP from IRAK, which can then transmit the IL1-induced signals.
UniProt Number:Q9H0E2
Gene ID:TOLLIP
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human TOLLIP. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human TOLLIP
