ST13(Suppression of Tumorigenicity 13), also known as P48 or HIP, is a protein that in humans is encoded by the ST13 gene.
ST13 is an abundant, highly conserved protein that binds the major cytosolic chaperones heat-shock protein 70-kD (HSP70) and HSP90 during an intermediate stage of steroid receptor assembly, but is absent from the mature receptor complex.
Zhang et al.
(1998) mapped the ST13 gene to chromosome 22q13 by fluorescence in situ hybridization.
They noted that colorectal, breast, and ovarian carcinomas frequently show loss of heterozygosity at this site.
Using a yeast 2-hybrid assay, Hohfeld et al.
(1995) showed that rat Hip bound Hsc70 (HSPA8).
One Hip oligomer bound the ATPase domains of at least 2 Hsc70 molecules, and binding was dependent on activation of the Hsc70 ATPase by Hsp40 (DNAJB1).
Hip stabilized the ADP-bound form of Hsc70, which had a high affinity for a test protein substrate.
Hohfeld et al.
(1995) concluded that HIP contributes to interactions of HSC70 with target proteins through its own chaperone activity.
UniProt Number:P50502
Gene ID:ST13
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human ST13. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human ST13, identical to the related mouse sequence.
