SNAP23(Synaptosomal-Associated Protein, 23-KD), also called SNAP23A, is a protein that in humans is encoded by the SNAP23 gene.
The SNAP23 gene has 8 exons, with the initiation codon located in exon 2.
The SNAP23 gene is mapped on 15q15.1-q15.2.
The SNAP23 cDNA encodes a 211-amino acid polypeptide with a predicted mass of 23 kD.
Its amino acid sequence is 59% identical to that of SNAP25.
Northern blot analysis revealed that SNAP23 is ubiquitously expressed.
SNAP23 is able to bind to multiple syntaxins as well as to multiple vesicle-associated membrane proteins.
After relocation, SNAP23 is required for exocytosis, implying a crucial role in promoting membrane fusion.
TIVAMP-containing vesicles were concentrated in the apical domain of epithelial cells.
STX3A and SNAP23 were codistributed at the apical plasma membrane, where they formed N-ethyl maleimide-dependent SNARE complexes with TIVAMP and cellubrevin.
SNAP23 is structurally and functionally similar to SNAP25 and binds tightly to multiple syntaxins and synaptobrevins/VAMPs.
It is an essential component of the high affinity receptor for the general membrane fusion machinery and is an important regulator of transport vesicle docking and fusion.
UniProt Number:O00161
Gene ID:SNAP23
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat SNAP23. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human SNAP23, different from the related rat and mouse sequences by three amino acids .
