SHC1(SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene.
SCOP classifies the 3D structure as belonging to the SH2 domain family.
By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.
(1994) assigned the SHC1 gene to 1q21.
Yulug et al.
(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21.
By the same method, an SHC-related sequence (SHCL1) was mapped to 17q21-q22.
By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.
(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1.
SHC1P1 is 85% identical to mouse Shc p66.
Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging.
Nemoto and Finkel (2002) concluded that there is an important functional relationship between forkhead proteins (e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are thought to be involved in the aging process in worms and mammals.
UniProt Number:P29353
Gene ID:SHC1
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human,mouse and rat SHC1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human SHC1, different from the related rat and mouse sequences by two amino acids.
