SDHB(Succinate Dehydrogenase Complex, Subunit B, iron sulfur protein), also known as iron-sulfur subunit of complex II (Ip) or SDH2, HOMOLOG OF, is a protein that in humans is encoded by the SDHB gene.
SDHB is one of four protein subunits forming succinate dehydrogenase, the other three being SDHA, SDHC and SDHD.
The SDHB subunit is connected to the SDHA subunit on the hydrophilic, catalytic end of the SDH complex.
The SDHB gene is mapped on 1p36.13.
The entire SDHB transcript is encoded by 8 exons within approximately 40 kb by Au et al(1995).
Pollard et al.(2005) stated that the nuclear-encoded Krebs cycle enzymes fumarate hydratase and succinate dehydrogenases like SDHB act as tumor suppressors, and germline mutations in these genes predispose individuals to leiomyomas and renal cancer and to paragangliomas, respectively.
In affected members of families with paragangliomas-4, mutations were identified in the SDHB gene .
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Staining of paraffin-fixed tissues requires boiling tissue sections in10mM citrate buffer, pH 6.0 for 20 minutes
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human SDHB. No crossreactivity with other proteins.Species Crossreactivity: rat. Species Sequence Homology: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa42-59, FAIYRWDPDKAGDKPHMQ, from human SDHB at the N-terminus, different from related rat sequence by one amino acid and mouse sequence by two amino acids.
