84-2099-16　RAB5A (Ras-related protein Rab-5A) 100ug　145119

特徴

• RAB5A(Ras-associated protein RAB5A), also called RAB5, is a protein that in humans is encoded by the RAB5A gene.
• RAB5 is a rate-limiting component of the machinery regulating the kinetics of membrane traffic in the early endocytic pathway.
• The RAB5A gene is mapped on 3p24.3.
• Tuberin functions as a RAB5GAP in vivo to negatively regulate RAB5-GTP activity in endocytosis.
• Ectopically expressed ALS2 localized with RAB5 and early endosome antigen-1 onto early endosomal compartments and stimulated the enlargement of endosomes in cultured cortical neurons.
• RAB5 is indispensable for a form of receptor tyrosine kinase-induced actin remodeling called circular ruffling.
• RAB5 signals to the actin cytoskeleton through RNTRE, a RAB5-specific GTPase-activating protein (GAP).
• RAB5 activity on phagosome membranes began to increase on disassembly of the actin coat encapsulating phagosomes.
• RAB5 activation was either continuous or repetitive for up to 10 minutes, but it ended before the collapse of engulfed apoptotic cells.
• Expression of a dominant-negative mutant of RAB5 delayed this collapse of apoptotic thymocytes, showing a role for RAB5 in phagosome maturation.
• UniProt Number:P20339
• Gene ID:RAB5A
• Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Handling:Store at -20˚C for one year.
• After reconstitution, store at 4˚C for one month.
• Can also be aliquoted and stored frozen at -20˚C for long term
• Avoid repeated freezing and thawing.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.