PHB(Prohibitin), also known as PHB1, is a protein that in humans is encoded by the PHB gene.
White et al.
(1991) mapped the PHB gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone.
By in situ hybridization, they localized the gene to 17q21.
Sato et al.
(1992) isolated the human homolog of the rat prohibitin gene and mapped it to 17q12-q21 by in situ hybridization.
Proliferation of tumor cells depends on new blood vessel formation (angiogenesis) that accompanies malignant progression.
Anticancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials.
Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand.
Furthermore, it is highly vascularized.
Rupnick et al.
(2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.
UniProt Number:P35232
Gene ID:PHB
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat PHB. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human RELB, identical to the related rat and mouse sequences.
