PRKDC (Protein Kinase DNA-Activated Catalytic Subunit), also called DNAPK, HYRC1, p350 or DNPK1, is an enzyme that in humans is encoded by the PRKDC gene.
DNA-PKcs belongs to the phosphatidylinositol 3-kinase-related kinase protein family.
Satoh et al.
(1997) mapped the MCM4 gene to 8q11.2 by FISH.
Based on the close proximity of the PRKDC and MCM4 genes, it was assumed that the PRKDC gene also maps to this location.
Anderson and Lees-Miller (1992) noted that DNA-PK had been shown in vitro to phosphorylate several transcription factors, suggesting that it functions in cell homeostasis by modulating transcription.
Daniel et al.
(1999) demonstrated that the PRKDC protein participates in retroviral DNA integration, which is catalyzed by the viral protein integrase.
UniProt Number:P78527
Gene ID:PRKDC
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human PRKDC. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human PRKDC.
