NSE (neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias.
The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway.
ENO2 gene contains 12 exons distributed over 9,213 nucleotides.
Human neurone-specific enolase is mapped to chromosome 12p13.
UniProt Number:P09104
Gene ID:ENO2
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity:Recognizes human, mouse and rat NSE. No crossreactivity with other proteins.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Calc Crossreactivity:Hu Mo Rt
Immunogen:A synthetic peptide corresponding to a sequence at the C-terminal of human NSE, identical to the related mouse and rat sequences.