NPM1(Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene.
The NPM1 gene maps to chromosome 5q35.
Chan et al.
(1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells.
Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level.
They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes.
Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
UniProt Number:P06748
Gene ID:NPM1
Applications:Suitable for use in Western Blot, Immunohistochemistry (Paraffin) and Immunocytochemistry.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat NPM1. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the N-terminal of human NPM1, identical to the related rat and mouse sequences.
