NOX4(NADPH oxidase 4), also called RENAL NADPH OXIDASE or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases.
Geiszt et al.
(2000) stated that the nucleotide sequence of RENOX matches that found in a genomic clone on chromosome 15.
In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to RENOX.
By FISH, Shiose et al.
(2001) mapped the NOX4 gene to chromosome 11q14.2-q21.
Geiszt et al.
(2000) found that NIH 3T3 fibroblasts overexpressing transfected RENOX showed increased production of superoxide and developed signs of cellular senescence.
They suggested that RENOX, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney.
By biochemical analysis of endogenous renal NOX4, Shiose et al.
(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.
UniProt Number:Q9JHI8
Gene ID:NOX4
Applications:Suitable for use in Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat NOX4. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of mouse NOX4, identical to the related rat sequence and different from the related human sequence by two amino acids.
