NLRP3(NLR FAMILY, PYRIN DOMAIN-CONTAINING 3),also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 (NOD-like receptor family, pryin domain containing 3) gene.
The NLRP3 gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes.
And the NLRP3 gene is mapped on 1q44.
NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD (ASC).
The encoded protein may play a role in the regulation of inflammation and apoptosis.
Mutation of the NALP3 nucleotide-binding domain reduced ATP binding, CASP1 activation, IL1B production, cell death, macromolecular complex formation, self-association, and association with ASC.
Consistent with an essential role for Nlrp3 inflammasomes in antifungal immunity, Gross et al.showed that Nlrp3-deficient mice are hypersusceptible to C.
albicans infection.
Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells.
The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutes is required for staining of formalin/parffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitite with 200ul sterile ddH2O.
Specificity：Recognizes human NLRP3. Species Crossreactivity: rat. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa12-31, RYLEDLEDVDLKKFMHLED at the N-terminal of human NLRP3, different from the related rat and mouse sequences by one amino acid. UniProt: Q96P20. Species Sequence Homology: mouse
