VGF (VGF, Nerve Growth Factor-Inducible), is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism.
Canu et al.
(1997) noted that rat Vgf encodes a predicted 70-kD polypeptide that shares similarities with the secretogranin/chromogranin family and is found in the secretory granules of subsets of neurons and endocrine cells.
By fluorescence in situ hybridization, Canu et al.
(1997) assigned the VGF gene to 7q22.
Canu et al.
(1997) demonstrated that the single-copy human VGF gene spans 6 kb of genomic DNA and contains 2 exons.
The entire VGF protein is encoded by exon 2, while exon 1 contains only 5-prime untranslated sequence.
The structural organization of the human gene is similar to that described for the rat VGF gene (Salton et al., 1991), and both the translated and the untranslated regions show a high degree of sequence homology to the rat gene.
UniProt Number:O15240
Gene ID:VGF
Applications:Suitable for use in Western Blot.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Handling:Store at -20˚C for one year.
After reconstitution, store at 4˚C for one month.
Can also be aliquoted and stored frozen at -20˚C for long term
Avoid repeated freezing and thawing.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Specificity：Recognizes human, mouse and rat VGF. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to a sequence at the C-terminal of human VGF, different from the related rat and mouse sequences by one amino acid.
